高速量化成像流式细胞仪Spectral Cell Sorter with High-Speed Cell Imaging BD FACSDiscover S8
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19/人使用者
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303/次机时次数
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1030.2/小时总时长
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0/次送样次数
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28/人收藏者
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收费标准
机时960元/小时 -
设备型号
FACSDiscover S8 -
当前状态
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管理员
陈心怡 18718204132,刘婕13760450981,孙大千 -
放置地点
卫光生命科学园1A栋二层207-A
- 仪器信息
- 预约资源
- 附件下载
- 公告
- 同类仪器
名称
高速量化成像流式细胞仪Spectral Cell Sorter with High-Speed Cell Imaging BD FACSDiscover S8
资产编号
SA2025080000026
型号
FACSDiscover S8
规格
产地
厂家
BD
所属品牌
BD
出产日期
购买日期
所属单位
细胞分析与免疫平台
使用性质
科研
所属分类
流式分析分选仪器
资产负责人
孙大千
联系电话
联系邮箱
chenxinyi@smart.org.cn,liujie@smart.org.cn,sundaqian@smart.org.cn
放置地点
卫光生命科学园1A栋二层207-A
- 主要规格&技术指标
- 主要功能及特色
- 样本检测注意事项
- 设备使用相关说明
主要规格&技术指标
一、光学参数
1）4激光（405/488/561/638nm)，56个荧光检测器，5个散射光检测器；
2）全光谱解析，荧光检测范围：410nm-860nm。
1.Optics Parameters
1)4 lasers（405/488/561/638nm), 56 fluorescence detectors, 5 scatter detectors;
2)Full spectrum profiling, covering 410nm-860nm.
二、成像参数
1）成像通道（488nm）：Light Loss、FSC、SSC、Image FL1 BP/534/46、Image FL2 BP/600/60、Image FL3 BP/788/225；
2）图像数据：偏心率、径向力矩、大小、相关度、质心、扩散度、总强度、最大强度、最长/最短力矩。
2.Imaging Parameters
1)Imaging channels (488nm): Light Loss, FSC, SSC, Image FL1 BP/534/46, Image FL2 BP/600/60 , Image FL3 BP/788/225;
2)Imaging features：Eccentricity, Radial moment, Size, Correlation, Delta center of mass, Diffusivity, Total intensity, Max intensity, Moment (long/short).
三、分选参数
1）喷嘴：85/100/130µm
85µm：1-6路分选；
100µm：1-4路分选；
130µm：1-2路分选。
2）收集方式
管式：1.5/2 mL离心管，5mL流式管；
板式：6-384孔板，载玻片。
3）分选速度≥15000 events/s。
3.Sorting Parameters
1) Nozzle: 85/100/130µm
85µm: 6-way sorting;
100µm: 4-way sorting;
130µm: 2-way sorting.
2) Sorting collection devices
Tube sorting: 1.5/2 mL centrifuge tubes, 5 mL 12×75-mm tubes;
Plate sorting: 6-384-well plates, microscope slide.
3) Sorting rate≥15000 events/s.
1）4激光（405/488/561/638nm)，56个荧光检测器，5个散射光检测器；
2）全光谱解析，荧光检测范围：410nm-860nm。
1.Optics Parameters
1)4 lasers（405/488/561/638nm), 56 fluorescence detectors, 5 scatter detectors;
2)Full spectrum profiling, covering 410nm-860nm.
二、成像参数
1）成像通道（488nm）：Light Loss、FSC、SSC、Image FL1 BP/534/46、Image FL2 BP/600/60、Image FL3 BP/788/225；
2）图像数据：偏心率、径向力矩、大小、相关度、质心、扩散度、总强度、最大强度、最长/最短力矩。
2.Imaging Parameters
1)Imaging channels (488nm): Light Loss, FSC, SSC, Image FL1 BP/534/46, Image FL2 BP/600/60 , Image FL3 BP/788/225;
2)Imaging features：Eccentricity, Radial moment, Size, Correlation, Delta center of mass, Diffusivity, Total intensity, Max intensity, Moment (long/short).
三、分选参数
1）喷嘴：85/100/130µm
85µm：1-6路分选；
100µm：1-4路分选；
130µm：1-2路分选。
2）收集方式
管式：1.5/2 mL离心管，5mL流式管；
板式：6-384孔板，载玻片。
3）分选速度≥15000 events/s。
3.Sorting Parameters
1) Nozzle: 85/100/130µm
85µm: 6-way sorting;
100µm: 4-way sorting;
130µm: 2-way sorting.
2) Sorting collection devices
Tube sorting: 1.5/2 mL centrifuge tubes, 5 mL 12×75-mm tubes;
Plate sorting: 6-384-well plates, microscope slide.
3) Sorting rate≥15000 events/s.
主要功能及特色
全光谱+成像+分选 Spectral + Imaging + Sorting
样本检测注意事项
一、推荐样本浓度为1x10^7 cells/mL。
1. Recommended sample concentration: 1×10^6 cells/mL.
二、上样前请用300目筛网过滤。
2. Please filter with a 300-mesh sieve before loading.
1. Recommended sample concentration: 1×10^6 cells/mL.
二、上样前请用300目筛网过滤。
2. Please filter with a 300-mesh sieve before loading.
设备使用相关说明
一、标准收费价格：960元/小时；最小收费时长为0.5小时。
1. The standard rate: 960 yuan/hour ; the minimum chargeable hours are 0.5 hours..
二、运行规则
1）预约仪器：最早可提前7天进行。
2）取消预约：至少需要提前2小时。
3）最小计费：最小计费时长0.5小时，不足0.5小时按0.5小时计算。
4）计费时长：总计费时长取“预约时间”和“实际使用时间”中的最早时刻作为开始时间，最晚时刻作为结束时间。（如若提前完成实验下机超过30分钟可释放机时）。【示例：预约9:00-10:00，使用9:30~10:20。总计费时长9:00~10:20共1.5小时，实际收费时长则为1.5小时。】
5）释放机时：至少需0.5小时及以上，且只能释放0.5小时的整数倍，不足0.5小时无法释放（释放机时操作：下机—手机佰能预约助手—服务—预约记录—对应记录点“释放”）【示例：预约9:00~10:00，9:36时下机则无法释放剩余的24min，9:26时下机后可释放0.5小时机时】【提醒：请提前进入释放界面或预留进入释放界面的时间，以免释放操作导致的延迟下机无法释放】。
2. Rules in Operation
1) Instrument Reservation: Reservations can be made up to 7 days in advance.
2) Cancellation: Cancellation must be made at least 2 hours in advance.
3) Minimum Billing: The minimum billing period is 0.5 hour; any usage less than 0.5 hour will be rounded up to 0.5 hour.
4) Billing Duration: The total billed duration is calculated by taking the earliest time between the "reserved time" and the "actual usage time" as the start time, and the latest time as the end time. (If you finish the experiment and release the instrument more than 30 minutes early, the unused time can be released.)
*Example: Reservation 9:00–10:00, actual usage 9:30–10:20. Total billed duration is from 9:00 to 10:20 = 1.5 hours, so the actual charged duration is 1.5 hours.*
5) Releasing Unused Time: At least 0.5 hour of unused time is required, and only integer multiples of 0.5 hour can be released; any remaining time less than 0.5 hour cannot be released. (How to release: after finishing, go to the mobile app "Baineng Reservation Assistant" → Services → Reservation Records → click "Release" on the corresponding record.)
Example: Reservation 9:00–10:00. If you finish at 9:36, the remaining 24 minutes cannot be released. If you finish at 9:26, you can release 0.5 hour of unused time.
Reminder: Please enter the release interface in advance or allow enough time to access it, to avoid being unable to release unused time due to delays caused by the release operation.
三、预约上机时间
新用户：请通过邮件或电话进行有限次数的预约，无法在预约网站自行预约机时。
初级用户：工作日 9:00-17:30。
中级用户：工作日 9:00-21:00，周末及法定节假日9:00-17:00。
高级用户：7天24小时。
3. Reservation Time
New users: Please make a limited number of reservations by email or phone. It is not possible to make your own reservations at the website.
Junior users: Weekdays 9:00-17:30.
Intermediate users: Weekdays 9:00-21:00, weekends and legal holidays 9:00-17:00.
Senior users: 24 hours a day, 7 days a week.
四、无菌分选：请提前一天联系工程师，以便提前进行无菌处理。如有问题欢迎进一步联系沟通。
4. Aseptic Sorting: Please contact the engineer one day in advance for aseptic processing. If there are any questions, welcome to contact us further.
1. The standard rate: 960 yuan/hour ; the minimum chargeable hours are 0.5 hours..
二、运行规则
1）预约仪器：最早可提前7天进行。
2）取消预约：至少需要提前2小时。
3）最小计费：最小计费时长0.5小时，不足0.5小时按0.5小时计算。
4）计费时长：总计费时长取“预约时间”和“实际使用时间”中的最早时刻作为开始时间，最晚时刻作为结束时间。（如若提前完成实验下机超过30分钟可释放机时）。【示例：预约9:00-10:00，使用9:30~10:20。总计费时长9:00~10:20共1.5小时，实际收费时长则为1.5小时。】
5）释放机时：至少需0.5小时及以上，且只能释放0.5小时的整数倍，不足0.5小时无法释放（释放机时操作：下机—手机佰能预约助手—服务—预约记录—对应记录点“释放”）【示例：预约9:00~10:00，9:36时下机则无法释放剩余的24min，9:26时下机后可释放0.5小时机时】【提醒：请提前进入释放界面或预留进入释放界面的时间，以免释放操作导致的延迟下机无法释放】。
2. Rules in Operation
1) Instrument Reservation: Reservations can be made up to 7 days in advance.
2) Cancellation: Cancellation must be made at least 2 hours in advance.
3) Minimum Billing: The minimum billing period is 0.5 hour; any usage less than 0.5 hour will be rounded up to 0.5 hour.
4) Billing Duration: The total billed duration is calculated by taking the earliest time between the "reserved time" and the "actual usage time" as the start time, and the latest time as the end time. (If you finish the experiment and release the instrument more than 30 minutes early, the unused time can be released.)
*Example: Reservation 9:00–10:00, actual usage 9:30–10:20. Total billed duration is from 9:00 to 10:20 = 1.5 hours, so the actual charged duration is 1.5 hours.*
5) Releasing Unused Time: At least 0.5 hour of unused time is required, and only integer multiples of 0.5 hour can be released; any remaining time less than 0.5 hour cannot be released. (How to release: after finishing, go to the mobile app "Baineng Reservation Assistant" → Services → Reservation Records → click "Release" on the corresponding record.)
Example: Reservation 9:00–10:00. If you finish at 9:36, the remaining 24 minutes cannot be released. If you finish at 9:26, you can release 0.5 hour of unused time.
Reminder: Please enter the release interface in advance or allow enough time to access it, to avoid being unable to release unused time due to delays caused by the release operation.
三、预约上机时间
新用户：请通过邮件或电话进行有限次数的预约，无法在预约网站自行预约机时。
初级用户：工作日 9:00-17:30。
中级用户：工作日 9:00-21:00，周末及法定节假日9:00-17:00。
高级用户：7天24小时。
3. Reservation Time
New users: Please make a limited number of reservations by email or phone. It is not possible to make your own reservations at the website.
Junior users: Weekdays 9:00-17:30.
Intermediate users: Weekdays 9:00-21:00, weekends and legal holidays 9:00-17:00.
Senior users: 24 hours a day, 7 days a week.
四、无菌分选：请提前一天联系工程师，以便提前进行无菌处理。如有问题欢迎进一步联系沟通。
4. Aseptic Sorting: Please contact the engineer one day in advance for aseptic processing. If there are any questions, welcome to contact us further.
预约资源
附件下载
公告
同类仪器
